NorthWest Building, Room 445.30
52 Oxford Street
Boston, MA 02115
Lab Size: Between 5 and 10
The Denic lab takes advantage of novel methodologies for studying large-scale genetic interactions in budding yeast as well as mass spectrometry-based characterization of natively-isolated protein complexes in order to identify the essential components required for several membrane-associated cellular processes. We then carry out targeted and systematic biochemical reconstitution strategies using the identified components in order to go from parts lists to functional and mechanistic insights.
Denic V, Dötsch V, Sinning I. (2012). ER Targeting and Insertion of Tail-Anchored Proteins by the GET Pathway. Cold Spring Harbor Review, (in press).
Denic V. (2012). A portrait of the GET pathway as a surprisingly complicated young man. Trends in Biochemical Sciences, Aug. 27 (Epub).
Wang F., Whynot A., Tung M., Denic V. (2011). The Mechanism of Tail-Anchored Protein Insertion into the ER Membrane.Molecular Cell; 43, 738-750.
Stefer S., Reitz S., Wang F., Wild K., Pang YY., Schwarz D, Bomke J, Hein C, Löhr F, Bernhard F, Denic V*., Dötsch V*., Sinning I*.Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex. Science; 2011 Aug. 5; 333(6043): 758-762. *co-corresponding authors.
Denic V. (2011). No Country for Old Misfolded Glycoproteins. Molecular Cell; 42, 715-717.
Wang F., Brown E. C., Mak G., Zhuang J., Denic V. (2010). A Chaperone Cascade Sorts Proteins for Post-Translational Membrane Insertion into the ER. Molecular Cell; 40, 159-171